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Prof. Dr. Ernst Wagner

Chair of Pharmaceutical Biotechnology

Affiliation

LMU Department of Pharmacy - Center for System-Based Drug Research and LMU Center for Nanoscience

Contact

Ludwig Maximilians University Munich
Department of Pharmacy - Center for Drug Research
Pharmaceutical Biotechnology
Butenandtstr. 5-13
D-81377 Munich

Phone: +49 (89) 2180 - 77841
Fax: +49 (89) 2180 - 77791

Website: http://www.cup.lmu.de/pb/aks/ewagner/members/prof-di-dr-ernst-wagner.html

Research Focus

Novel molecular therapeutics like siRNA, microRNAs or antagomiRs require potent extracellular and intracellular delivery devices to be effective antitumor agents.

For this purpose we design programmed nanoagents (www.nano-initiative-munich.de) which recognize tumor cells by specific targeting mechanisms, invade the cells and release the bioactive therapeutic nucleic acid in the cytosol of target cells.

Potential Project:

Receptor-targeted, nucleic acid loaded nanoagents based on precise synthetic carrier polymers (see Schaffert, Angewandte Chemistry 2011; Nie, Biomaterials 2011) will be tested in tumor cell cultures for manipulation of cancer cells in a specific fashion (see for example Schaffert Pharm. Research 2011). Folate or tyrosine kinase receptor ligands will be applied for targeting, siRNAs and miRNAs manipulating the cell-cycle or phenotype of tumors will be applied.

Primary Techniques: Nanoparticle formation and characterization, delivery into cultured tumor cells, analysis of gene expression patterns, RT-QPCR, fluorescence microscopy, immunohistochemistry, flow cytometry, cell cycle analysis.

Model Organism(s): tumor cell lines, mouse tumor models