LMU Medical Faculty, Adolf Butenandt Institute, Department of Molecular Biology, Yeast Chromatin
Adolf Butenandt Institute
Chair of Molecular Biology
+49 (89) 2180 - 75435
Fax: +49 (89) 2180 - 75425
The Korber group studies the mechanisms of nucleosome positioning and remodeling. Nucleosomes are the fundamental building blocks of chromatin, which organize eukaryotic DNA in the nucleus. A major fraction of nucleosomes is not distributed randomly, but occupies defined positions along the DNA sequence. Such nucleosome positioning regulates the accessibility of DNA. For example, a transcription factor binding site may be intranucleosomal and less accessible or resides in a nucleosome free region and be readily bound by its corresponding factor. On one hand, the aim is to understand what determines nucleosome positioning. On the other hand, the Korber lab studies how nucleosomes are rendered accessible, for example, during chromatin opening at promoters upon gene induction. The group uses the unicellular yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe as model systems and combines their powerful genetic tools with biochemical techniques.
Primary Technique(s): genetic tools (molecular cloning in E. coli and yeasts), biochemical techniques (in vitro reconstitution with extracts and purified factors)
Model Organism(s): unicellular yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe